Immunofluorescence learning resources

[mickelj7]

I have a lot of experience in histology and slide staining and I'm pretty new to IF staining. I'm looking for information and learning resources on IF. Webinars, seminars, workshops, online learning tools, I am open to it all.
Thank you all in advance.

[radtkea]

Hi @mickelj7,

Thank you very much for your excellent question!

We have several videos on the IBEX Imaging Community. You can find general talks as well as tutorials for specific steps.

I am partial to this review. Here we summarized methods, challenges, and included lots of resources to aid with multiplexed imaging in the Supplementary Tables. I recommend you check out the Supplementary Information (See below).

Webinars:
Labroots has a lot of free talks related to spatial biology and immunofluorescence microscopy.

Nature hosted a conference on spatial biology.

Workshops:
We have this course in Germany. We will be discussing a wide variety of topics. Would love for you to join us.

Online tutorials: Lots of vendors have great content on their sites.
Leica posts articles on Science Lab.

BINA has several talks related to microscopy.

Can I ask what kind of content would be particularly helpful? For example, are you interested in how to select antibodies, validate antibodies and panels, conjugate antibodies, and acquire multiplexed images? Would you prefer online, in-person, or hybrid content?

@zivy We should revisit our IBEX hackathon topic:)

Best wishes,
Andrea

[radtkea]

Check out this seminar from members of our IBEX community.

It is March 6th at 10 am ET.

Best wishes,
Andrea