diff --git a/mysql-test/suite/vcol/r/vcol_misc.result b/mysql-test/suite/vcol/r/vcol_misc.result
index 1a16fd1a3944e..4bade1bfa4ee4 100644
--- a/mysql-test/suite/vcol/r/vcol_misc.result
+++ b/mysql-test/suite/vcol/r/vcol_misc.result
@@ -591,3 +591,21 @@ DROP TABLE t;
 #
 # End of 10.5 tests
 #
+#
+# MDEV-28610 Assertion `marked_for_read()' failed upon range select with virtual column in index
+#
+CREATE TABLE t (
+a INT UNSIGNED NOT NULL DEFAULT 0,
+b INT AS (a) VIRTUAL,
+c TIME,
+d INT,
+e DATE,
+f VARCHAR(1024),
+PRIMARY KEY(c),
+KEY(c,b,d,f(64))
+);
+INSERT INTO t (c) VALUES ('00:00:00'),('00:00:04');
+SELECT c FROM t WHERE c BETWEEN '00:00:00' AND '00:00:00' ORDER BY d;
+c
+00:00:00
+DROP TABLE t;
diff --git a/mysql-test/suite/vcol/t/vcol_misc.test b/mysql-test/suite/vcol/t/vcol_misc.test
index 2709ff5b46535..12993bb489f58 100644
--- a/mysql-test/suite/vcol/t/vcol_misc.test
+++ b/mysql-test/suite/vcol/t/vcol_misc.test
@@ -560,3 +560,23 @@ DROP TABLE t;
 --echo #
 --echo # End of 10.5 tests
 --echo #
+
+--echo #
+--echo # MDEV-28610 Assertion `marked_for_read()' failed upon range select with virtual column in index
+--echo #
+
+CREATE TABLE t (
+  a INT UNSIGNED NOT NULL DEFAULT 0,
+  b INT AS (a) VIRTUAL,
+  c TIME,
+  d INT,
+  e DATE,
+  f VARCHAR(1024),
+  PRIMARY KEY(c),
+  KEY(c,b,d,f(64))
+);
+INSERT INTO t (c) VALUES ('00:00:00'),('00:00:04');
+SELECT c FROM t WHERE c BETWEEN '00:00:00' AND '00:00:00' ORDER BY d;
+
+# Cleanup
+DROP TABLE t;
diff --git a/sql/sql_select.cc b/sql/sql_select.cc
index 70785f531bb00..626a9bf27a7e9 100644
--- a/sql/sql_select.cc
+++ b/sql/sql_select.cc
@@ -32822,7 +32822,7 @@ void JOIN::init_join_cache_and_keyread()
           tuple.
       */
       if (!(table->file->index_flags(table->file->keyread, 0, 1) & HA_CLUSTERED_INDEX))
-        table->mark_index_columns(table->file->keyread, table->read_set);
+        table->mark_index_columns_for_read(table->file->keyread);
     }
     bool init_for_explain= false;
 
diff --git a/sql/table.cc b/sql/table.cc
index 2b283fd93e1b8..132680299e91e 100644
--- a/sql/table.cc
+++ b/sql/table.cc
@@ -7664,7 +7664,7 @@ inline void TABLE::mark_index_columns_no_reset(uint index, MY_BITMAP *bitmap)
 }
 
 
-inline void TABLE::mark_index_columns_for_read(uint index)
+void TABLE::mark_index_columns_for_read(uint index)
 {
   do_mark_index_columns(this, index, read_set, true);
 }