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<!DOCTYPE html>
<html lang="en">
<head>
<meta charset="UTF-8">
<title>Metabolomics Workbench File Validator</title>
<link rel="stylesheet" href="styles/styles.css"/>
</head>
<body class="background">
<div>
<h1><a href="https://MoseleyBioinformaticsLab.github.io/mwFileStatusWebsite/" style="text-decoration:none;color:white;">Metabolomics Workbench File Validator</a></h1>
<p>
Last Updated: 2026-03-01 08:09:13.500362
</p>
</div>
<div>
<h2>Statistics</h2>
<p>
Number of Studies: 8<br>
Number of Analyses: 9<br>
</p>
<h3>Validation Statistics</h3>
<div class="stats__grid">
<div class="stats__grid__item">Status</div>
<div class="stats__grid__item">mwTab</div>
<div class="stats__grid__item">JSON</div>
<a href="https://7.github.io/MoseleyBioinformaticsLab/passing" class="stats__grid__item brightgreen">Passing</a>
<div class="stats__grid__item">0</div>
<div class="stats__grid__item">0</div>
<a href="https://7.github.io/MoseleyBioinformaticsLab/passing" class="stats__grid__item yellow">Warnings Only</a>
<div class="stats__grid__item">0</div>
<div class="stats__grid__item">0</div>
<a href="https://7.github.io/MoseleyBioinformaticsLab/validation_error" class="stats__grid__item orange">Validation Error</a>
<div class="stats__grid__item">0</div>
<div class="stats__grid__item">2</div>
<a href="https://7.github.io/MoseleyBioinformaticsLab/parsing_error" class="stats__grid__item red">Parsing Error</a>
<div class="stats__grid__item">0</div>
<div class="stats__grid__item">0</div>
<a href="https://7.github.io/MoseleyBioinformaticsLab/missing" class="stats__grid__item brightred">Missing</a>
<div class="stats__grid__item">9</div>
<div class="stats__grid__item">7</div>
</div>
<p></p>
<div class="stats__grid">
<div class="stats__grid__item">Validation Error Type</div>
<div class="stats__grid__item">mwTab</div>
<div class="stats__grid__item">JSON</div>
<a href="https://MoseleyBioinformaticsLab.github.io/mwFileStatusWebsite/value" class="stats__grid__item orange">Value Error</a>
<div class="stats__grid__item">0</div>
<div class="stats__grid__item">2</div>
<a href="https://MoseleyBioinformaticsLab.github.io/mwFileStatusWebsite/consistency" class="stats__grid__item orange">Consistency Error</a>
<div class="stats__grid__item">0</div>
<div class="stats__grid__item">0</div>
<a href="https://MoseleyBioinformaticsLab.github.io/mwFileStatusWebsite/format" class="stats__grid__item orange">Format Error</a>
<div class="stats__grid__item">0</div>
<div class="stats__grid__item">1</div>
</div>
<h3>mwTab vs JSON Comparison Statistics</h3>
<div class="compare__stats__grid">
<div class="stats__grid__item">Status</div>
<div class="stats__grid__item">Count</div>
<div class="stats__grid__item brightgreen">Consistent</div>
<div class="stats__grid__item">0</div>
<div class="stats__grid__item orange">Inconsistent</div>
<div class="stats__grid__item">0</div>
<div class="stats__grid__item lightgrey">Not Checked</div>
<div class="stats__grid__item">9</div>
</div>
</div>
<div>
<h2>File Status</h2>
<div class="grid header">
<input type="checkbox" id="study_grid_item0" class="study_checkbox"/>
<label for="study_grid_item0" href="#ST001385" class="study__grid__item">ST001385: Urine Metabolomics - Icahn School of Medicine at Mount Sinai - Petrick, Lauren</label>
<div class="grid__description" style="height:8em;max-height:8">
<div class="desc__grid">
<div class="desc__grid__item">STUDY_TITLE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Urine Metabolomics</div>
<div class="desc__grid__item">STUDY_SUMMARY</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">To perform an untargeted metabolomics analysis of urine samples, matrix blanks and quality control samples. The metabolomics approach will be performed using both reverse phase (RP) and HILIC chromatography (ZHP) separations coupled to high-resolution mass spectrometry. Samples were received and stored at -80°C until processing. In total, 315 samples had sufficient sample volume for metabolomics analysis.</div>
<div class="desc__grid__item">INSTITUTE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Icahn School of Medicine at Mount Sinai</div>
<div class="desc__grid__item">LAST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Petrick</div>
<div class="desc__grid__item">FIRST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Lauren</div>
<div class="desc__grid__item">ADDRESS</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Department of Environmental Medicine and Public Health, Atran Building 3rd floor, 101st St. between Madison and 5th Ave, New York, New York, 10029, USA</div>
<div class="desc__grid__item">EMAIL</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">lauren.petrick@mssm.edu</div>
<div class="desc__grid__item">PHONE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">212 241 7351</div>
</div>
</div>
</div>
<div class="grid">
<input type="checkbox" id="analysis_grid_item1" class="analysis_checkbox"/>
<label for="analysis_grid_item1" href="#AN002312" class="analysis__grid__item brightred">AN002312</label>
<div class="grid__description">
<div>
<span>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN002312/mwtab/txt" target="_blank">txt</a>
</div>
<div class="shieldRight brightred">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/validation_logs/AN002312_txt.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN002312/mwtab/json" target="_blank">json</a>
</div>
<div class="shieldRight orange">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/validation_logs/AN002312_json.log" target="_blank">Validation Error</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN002312/mwtab/comparison" target="_blank">comparison</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/validation_logs/AN002312_comparison.log" target="_blank">Not Checked</a>
</div>
</div>
</span>
</div>
<br>
<div class="desc__grid">
<div class="desc__grid__item">ANALYSIS_ID</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">AN002312</div>
</div>
</div>
</div> <br> <div class="grid header">
<input type="checkbox" id="study_grid_item1" class="study_checkbox"/>
<label for="study_grid_item1" href="#ST003104" class="study__grid__item">ST003104: Metabolomics studies on human cardiac samples - University of Sydney - Koay, Yen Chin</label>
<div class="grid__description" style="height:13em;max-height:13">
<div class="desc__grid">
<div class="desc__grid__item">STUDY_TITLE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Metabolomics studies on human cardiac samples</div>
<div class="desc__grid__item">STUDY_SUMMARY</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Targeted metabolomics was performed to measure polar metabolites in both positive and negative ionization mode on left ventricular tissue acquired from pre-mortem healthy donor hearts as classified by formal pathological examination and stored at the Sydney Heart Bank. The minimum number of observations for young (age ≤ 25 years) and old (age ≥ 50 years) cohorts using liquid chromatography-tandem mass spectrometry (LC-MS/MS).</div>
<div class="desc__grid__item">INSTITUTE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">University of Sydney</div>
<div class="desc__grid__item">DEPARTMENT</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Medicine and Health</div>
<div class="desc__grid__item">LAST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Koay</div>
<div class="desc__grid__item">FIRST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Yen Chin</div>
<div class="desc__grid__item">ADDRESS</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Charles Perkin Centre</div>
<div class="desc__grid__item">EMAIL</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">yen.koay@sydney.edu.au</div>
<div class="desc__grid__item">PHONE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">+61486275851</div>
<div class="desc__grid__item">NUM_GROUPS</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">2</div>
<div class="desc__grid__item">TOTAL_SUBJECTS</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">25</div>
<div class="desc__grid__item">NUM_MALES</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">15</div>
<div class="desc__grid__item">NUM_FEMALES</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">10</div>
</div>
</div>
</div>
<div class="grid">
<input type="checkbox" id="analysis_grid_item2" class="analysis_checkbox"/>
<label for="analysis_grid_item2" href="#AN005082" class="analysis__grid__item brightred">AN005082</label>
<div class="grid__description">
<div>
<span>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005082/mwtab/txt" target="_blank">txt</a>
</div>
<div class="shieldRight brightred">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/validation_logs/AN005082_txt.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005082/mwtab/json" target="_blank">json</a>
</div>
<div class="shieldRight orange">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/validation_logs/AN005082_json.log" target="_blank">Validation Error</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005082/mwtab/comparison" target="_blank">comparison</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/validation_logs/AN005082_comparison.log" target="_blank">Not Checked</a>
</div>
</div>
</span>
</div>
<br>
<div class="desc__grid">
<div class="desc__grid__item">ANALYSIS_ID</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">AN005082</div>
</div>
</div>
</div> <br> <div class="grid header">
<input type="checkbox" id="study_grid_item2" class="study_checkbox"/>
<label for="study_grid_item2" href="#ST003113" class="study__grid__item">ST003113: Inhibition of Asparagine Synthetase Effectively Retards Polycystic Kidney Disease Progression, investigated with targeted tracing metabolomics analysis in MEF cells using 15N2-glutamine. - San Raffaele University - Stefanoni, Davide</label>
<div class="grid__description" style="height:8em;max-height:8">
<div class="desc__grid">
<div class="desc__grid__item">STUDY_TITLE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Inhibition of Asparagine Synthetase Effectively Retards Polycystic Kidney Disease Progression, investigated with targeted tracing metabolomics analysis in MEF cells using 15N2-glutamine.</div>
<div class="desc__grid__item">STUDY_SUMMARY</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Polycystic Kidney Disease (PKD) is a genetic disorder characterized by bilateral cyst formation. We showed that PKD cells and kidneys display metabolic alterations, including the Warburg effect and glutaminolysis, sustained in vitro by the enzyme asparagine synthetase (ASNS). Here, we used antisense oligonucleotides (ASO) against Asns in orthologous and slowly progressive PKD murine models and show that treatment leads to a drastic reduction of total kidney volume (measured by MRI) and a prominent rescue of renal function in the mouse. Mechanistically, the upregulation of an ATF4-ASNS axis in PKD is driven by the amino acid response (AAR) branch of the integrated stress response (ISR). Metabolic profiling of PKD or control kidneys treated with Asns-ASO or Scr-ASO revealed major changes in the mutants, several of which are rescued by Asns silencing in vivo. Indeed, ASNS drives glutamine-dependent de novo pyrimidine synthesis and proliferation in cystic epithelia. Notably, while several metabolic pathways were completely corrected by Asns-ASO, glycolysis was only partially restored. Accordingly, combining the glycolytic inhibitor 2DG with Asns-ASO further improved efficacy. Our studies identify a new therapeutic target and novel metabolic vulnerabilities in PKD. Of interest, in these tracing studies we could confirm that the pyrimidine biosynthesis pathway is increased and rescued by silencing of Asns.</div>
<div class="desc__grid__item">INSTITUTE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">San Raffaele University</div>
<div class="desc__grid__item">LAST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Stefanoni</div>
<div class="desc__grid__item">FIRST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Davide</div>
<div class="desc__grid__item">ADDRESS</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Via Olgettina 58, Milan, Milan, 20132, Italy</div>
<div class="desc__grid__item">EMAIL</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">stefanoni.davide@hsr.it</div>
<div class="desc__grid__item">PHONE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">+393337686005</div>
</div>
</div>
</div>
<div class="grid">
<input type="checkbox" id="analysis_grid_item3" class="analysis_checkbox"/>
<label for="analysis_grid_item3" href="#AN005098" class="analysis__grid__item brightred">AN005098</label>
<div class="grid__description">
<div>
<span>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005098/mwtab/txt" target="_blank">txt</a>
</div>
<div class="shieldRight brightred">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/validation_logs/AN005098_txt.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005098/mwtab/json" target="_blank">json</a>
</div>
<div class="shieldRight brightred">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/validation_logs/AN005098_json.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005098/mwtab/comparison" target="_blank">comparison</a>
</div>
<div class="shieldRight lightgrey">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/validation_logs/AN005098_comparison.log" target="_blank">Not Checked</a>
</div>
</div>
</span>
</div>
<br>
<div class="desc__grid">
<div class="desc__grid__item">ANALYSIS_ID</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">AN005098</div>
</div>
</div>
<input type="checkbox" id="analysis_grid_item4" class="analysis_checkbox"/>
<label for="analysis_grid_item4" href="#AN005099" class="analysis__grid__item brightred">AN005099</label>
<div class="grid__description">
<div>
<span>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005099/mwtab/txt" target="_blank">txt</a>
</div>
<div class="shieldRight brightred">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/validation_logs/AN005099_txt.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
<a href="https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005099/mwtab/json" target="_blank">json</a>
</div>
<div class="shieldRight brightred">
<a href="https://raw.githubusercontent.com/MoseleyBioinformaticsLab/mwFileStatusWebsite/master/validation_logs/AN005099_json.log" target="_blank">Missing/Blank</a>
</div>
</div>
<div class="shield">
<div class="shieldLeft grey">
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<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">AN005099</div>
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<label for="study_grid_item3" href="#ST003389" class="study__grid__item">ST003389: Disruption of Glucose Homeostasis by Bacterial Infection Orchestrates Host Innate Immunity Through NAD+/NADH Balance - Northwest A&F University - Xu, Lei</label>
<div class="grid__description" style="height:8em;max-height:8">
<div class="desc__grid">
<div class="desc__grid__item">STUDY_TITLE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Disruption of Glucose Homeostasis by Bacterial Infection Orchestrates Host Innate Immunity Through NAD+/NADH Balance</div>
<div class="desc__grid__item">STUDY_SUMMARY</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Metabolic reprogramming is crucial for activating innate immunity in macrophages, and the accumulation of immunometabolites is thought essential for effective defense against infection. The NAD+/NADH redox couple serves as a critical node that integrates metabolic pathways and signaling events, but how this metabolite couple engages macrophage activation remains unclear. Here, we showed that the NAD+/NADH ratio serves as a molecular signal that regulates proinflammatory responses and type I interferon (IFN) responses divergently. Salmonella Typhimurium infection led to a decreased NAD+/NADH ratio by inducing the accumulation of NADH. Further investigation showed that an increased NAD+/NADH ratio correlates to attenuated proinflammatory responses and enhanced type I IFN responses. Conversely, a decreased NAD+/NADH ratio is linked to intensified proinflammatory responses and restrained type I IFN responses. These results showed the NAD+/NADH ratio is an essential cell-intrinsic factor that orchestrates innate immunity, which enhanced our understanding of how metabolites fine-tune innate immunity.</div>
<div class="desc__grid__item">INSTITUTE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Northwest A&F University</div>
<div class="desc__grid__item">LAST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Xu</div>
<div class="desc__grid__item">FIRST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Lei</div>
<div class="desc__grid__item">ADDRESS</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Yangling, Xinong Road, Xiyang, 712100, China</div>
<div class="desc__grid__item">EMAIL</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">xulei@nwafu.edu.cn</div>
<div class="desc__grid__item">PHONE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">18149321513</div>
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<div class="desc__grid__item">ANALYSIS_ID</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">AN005557</div>
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<input type="checkbox" id="study_grid_item4" class="study_checkbox"/>
<label for="study_grid_item4" href="#ST003687" class="study__grid__item">ST003687: Cross-sectional salivary metabolomics of the Fin-HIT corhort - Ghent University - callemeyn, emile</label>
<div class="grid__description" style="height:10em;max-height:10">
<div class="desc__grid">
<div class="desc__grid__item">STUDY_TITLE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Cross-sectional salivary metabolomics of the Fin-HIT corhort</div>
<div class="desc__grid__item">STUDY_SUMMARY</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">This study is part of a multi-part study, including salivary metabolomics of 4 patient cohorts.</div>
<div class="desc__grid__item">INSTITUTE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Ghent University</div>
<div class="desc__grid__item">DEPARTMENT</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Department of Translational Physiology, Infectiology and Public Health</div>
<div class="desc__grid__item">LABORATORY</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Laboratory of Integrative Metabolomics</div>
<div class="desc__grid__item">LAST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">callemeyn</div>
<div class="desc__grid__item">FIRST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">emile</div>
<div class="desc__grid__item">ADDRESS</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Salisburylaan 133, 9820 Merelbeke, Belgium</div>
<div class="desc__grid__item">EMAIL</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">emile.callemeyn@ugent.be</div>
<div class="desc__grid__item">PHONE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">0499976319</div>
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<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">AN006051</div>
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<label for="study_grid_item5" href="#ST003744" class="study__grid__item">ST003744: None - None - None, None</label>
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<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">AN006148</div>
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<label for="study_grid_item6" href="#ST003819" class="study__grid__item">ST003819: Polar metabolite profiling of SLC31A1 knockout cells compared to intergenic sgRNA containing cells - Boston Childrens Hospital - Wong, Alan</label>
<div class="grid__description" style="height:9em;max-height:9">
<div class="desc__grid">
<div class="desc__grid__item">STUDY_TITLE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Polar metabolite profiling of SLC31A1 knockout cells compared to intergenic sgRNA containing cells</div>
<div class="desc__grid__item">STUDY_SUMMARY</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">To investigate the effects of SLC31A1 knockout on steady state metabolite levels, SEM (acute lymphoblastic leukaemia cell line) cells containing either a SLC31A1-targeting sgRNA (two different guides, guide #2 or guide #4) or a intergenic -targetting sgRNA (i.e. a control sgRNA, "Intergenic1") and Cas9 were FACS-purified (Fluorescence Activated Cell Sorting) based on SLC31A1 expression. Cells were then cultured in RPMI-1640 containing 10% FBS and penicillin and streptomycin in 6-well plates, and then collected for metabolomics. SLC31A1 knockout cells were found to exhibit profound electron transport chain inhibition, resulting in accumulation of carbamoyl aspartic acid/ureidosuccinic acid, and depletion of aspartate, as well as broad changes in nucleotide mono, di and triphosphate levels.</div>
<div class="desc__grid__item">INSTITUTE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Boston Childrens Hospital</div>
<div class="desc__grid__item">LAST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Wong</div>
<div class="desc__grid__item">FIRST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Alan</div>
<div class="desc__grid__item">ADDRESS</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">300 Longwood Avenue</div>
<div class="desc__grid__item">EMAIL</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">alan.wong@childrens.harvard.edu</div>
<div class="desc__grid__item">PHONE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">(617) 355-7433</div>
<div class="desc__grid__item">NUM_GROUPS</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">4</div>
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<label for="analysis_grid_item8" href="#AN006279" class="analysis__grid__item brightred">AN006279</label>
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<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">AN006279</div>
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<label for="study_grid_item7" href="#ST003999" class="study__grid__item">ST003999: Measuring PEMT activity in HCMV infected fibroblasts using 13C-methionine. - University of Arizona - Kline, Ian</label>
<div class="grid__description" style="height:11em;max-height:11">
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<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Measuring PEMT activity in HCMV infected fibroblasts using 13C-methionine.</div>
<div class="desc__grid__item">STUDY_SUMMARY</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">HCMV promotes the levels of PC lipids in infection. The Kennedy pathway is the canonical host pathway for de novo PC lipid synthesis. Host cells encode alternative pathways for PC synthesis such as the Phosphatidylethanolamine N-methyltransferase (PEMT) pathway. Here, we tested if HCMV promotes metabolic activity in the PEMT pathway. We used 13C-methionine to track the metabolism of labeled tracer molecule into the head group of PC lipids thus, measuring PEMT pathway activity. We find that HCMV and uninfected cells exhibit similarly low PEMT metabolic activity and percentage of labeled PCs. Moreover, when cells were engineered to overexpress the host PEMT enzyme, PC labeling via PEMT increased when expression was induced with doxycycline. Overexpressing GFP and feeding labeled molecule resulted in labeling similar to mock and infected labeling percentages, <5%. Thus, PEMT is not an active pathway in the primary human fibroblasts used in our studies and HCMV infection does not increase metabolic activity of PEMT to synthesize PCs.</div>
<div class="desc__grid__item">INSTITUTE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">University of Arizona</div>
<div class="desc__grid__item">DEPARTMENT</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Immunobiology</div>
<div class="desc__grid__item">LABORATORY</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">John G. Purdy, PhD</div>
<div class="desc__grid__item">LAST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Kline</div>
<div class="desc__grid__item">FIRST_NAME</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">Ian</div>
<div class="desc__grid__item">ADDRESS</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">1657 E Helen St, Tucson, AZ 85721</div>
<div class="desc__grid__item">EMAIL</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">ikline@arizona.edu</div>
<div class="desc__grid__item">PHONE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">5209092596</div>
<div class="desc__grid__item">SUBMIT_DATE</div>
<div class="desc__grid__item" style="white-space:nowrap;overflow:hidden;text-overflow:ellipsis;width:calc(100%);">2025-06-09</div>
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