diff --git a/synthtool/languages/node_mono_repo.py b/synthtool/languages/node_mono_repo.py
index 46cfd3bc5..d4e36cd99 100644
--- a/synthtool/languages/node_mono_repo.py
+++ b/synthtool/languages/node_mono_repo.py
@@ -482,6 +482,7 @@ def walk_through_owlbot_dirs(dir: Path, search_for_changed_files: bool):
     packages_to_exclude = [
         r"packages/gapic-node-processing/templates/bootstrap-templates",
         r"node_modules",
+        r"core/generator/gapic-generator-typescript/test-fixtures",
     ]
     if search_for_changed_files:
         try:
diff --git a/tests/test_node_mono_repo.py b/tests/test_node_mono_repo.py
index c9e7880a1..fdb2dd08b 100644
--- a/tests/test_node_mono_repo.py
+++ b/tests/test_node_mono_repo.py
@@ -509,6 +509,49 @@ def test_walk_through_owlbot_dirs_handwritten(mock_subproc_popen):
         assert any(re.search("handwritten/dlp", d) for d in owlbot_dirs)
 
 
+@patch("subprocess.run")
+def test_walk_through_owlbot_dirs_excluded(mock_subproc_popen):
+    process_mock = Mock()
+    attrs = {"communicate.return_value": ("output", "error")}
+    process_mock.configure_mock(**attrs)
+    mock_subproc_popen.return_value = process_mock
+
+    with util.copied_fixtures_dir(
+        FIXTURES / "nodejs_mono_repo_with_staging"
+    ) as workdir:
+        excluded_dir = (
+            workdir
+            / "core"
+            / "generator"
+            / "gapic-generator-typescript"
+            / "test-fixtures"
+            / "speech"
+        )
+        excluded_dir.mkdir(parents=True)
+        (excluded_dir / ".OwlBot.yaml").touch()
+
+        regular_dir = workdir / "core" / "generator" / "gapic-generator-typescript"
+        regular_dir.mkdir(parents=True, exist_ok=True)
+        (regular_dir / ".OwlBot.yaml").touch()
+
+        owlbot_dirs = node_mono_repo.walk_through_owlbot_dirs(
+            workdir, search_for_changed_files=False
+        )
+
+        assert not mock_subproc_popen.called
+        assert any(
+            re.search("core/generator/gapic-generator-typescript$", d)
+            for d in owlbot_dirs
+        )
+        assert not any(
+            re.search(
+                "core/generator/gapic-generator-typescript/test-fixtures/speech",
+                d,
+            )
+            for d in owlbot_dirs
+        )
+
+
 @patch("subprocess.run")
 def test_walk_through_owlbot_dirs_no_staging(mock_subproc_popen):
     process_mock = Mock()