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Trajectory analysis of myeloid cells in kidney allograft rejection

This repository contains the code to generate the results and figures from

A recipient-derived macrophages subset induces kidney allograft rejection through CXCL10 secretion and LILRB2 signaling

Alexis Varin‡, Jovanne Palvair‡, Lennie Messager‡, Jamal Bamoulid, Yacine Benchikh, Jasper Callemeyn, Mélanie Chaintreuil, Ludivine Dal Zuffo, Didier Ducloux, Imane Farhat, Mathieu Legendre, Laurent Martin, Florian Renosi, Xavier Roussel, Thibaut Vaulet, Maarten Naesens, Claire Tinel and Baptiste Lamarthée

‡ These authors contributed equally

Preprint: https://doi.org/10.1101/2025.04.21.25324260

Description

The Jupyter Notebook in this repository offers a complete step-by-step walkthrough of the computational workflow developped to determine the patterns at the single-cell resolution governing the differentiation of blood-circulating monocytes into tissue-infiltrating monocytes and finally macrophages, with an emphasis on elucidating the key features leading to the emergence of the pro-inflammatory macrophages secreting CXCL10 identified as central in driving kidney allograft rejection.

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Source code for unsupervised differentiation trajectory analysis of myeloid cells in kidney allograft rejection using slingshot, tradeSeq and RightOmicsTools packages from Varin et al. manuscript entitled "A recipient-derived macrophages subset induces kidney allograft rejection through CXCL10 secretion and LILRB2 signaling"

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